What I noticed was that while testing Avast, I run some samples that avast did not detect. Hardened mode was set at aggressive but all of them run normally without any hardened mode notification. One or two days before, Avast hardened mode worked successfully with every undetected sample (it blocked) in such situation with different samples. Both sets of samples were from malware hub here at MalwareTips.
I don't know if the virtual box I used had to do with the problem but CIS worked ok with all those samples in same environment and I cannot believe the samples avast did not block were special or something.
Maybe the whole thing is a bit silly and it may not represent something in real world, but I was worried by this incident. Think about the fact that Avast might be that buggy, at least the hardened mode!